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Thin Layer Chromatography

 

Silica gel with calcium sulphate (CaSO4 ½ H2O) as binder is by far the most extensively used adsorbent in Thin Layer Chromatography because of the excellent separation it affords. When coated on a plate in the form of thin layer it serves as a versatile solid stationary phase for adsorption and partition chromatography.

 

Range of products in Thin Layer Chromatography

a

Silica Gel TLC `H’ without binder

b

Silica Gel HF - 254 with fluorescent indicator F – 254

c

Silica Gel TLC `G’ with binder i.e. CaSO4 ½ H2O

d

Silica Gel GF – 254 with fluorescent indicator F – 254

 
APPLICATIONS
 

Silica Gel - `G’ can be successfully employed in both qualitative and quantitative analysis of alkaloids, amino acids, oils and fats, vitamins, plant pigments, drugs and sugars.

 

Specification of Silica Gel – Thin Layer Chromatography

Sr. No.

Properties

Unit

Grade

1

Grades

 

H

HF – 254

G

GF – 254

2

PH value (10% water Suspension)

 

7 ± 0.5

7 ± 0.5

7 ± 0.5

7 ± 0.5

3

Iron, as Fe

ppm, max.

300

200

300

200

4

Chloride, as Cl

ppm, max.

300

200

300

200

5

CaSO4 0.5 H2O

%

----

---

13.0 ± 0.5

13.0 ± 0.5

6

Suitability Test (Plate)

----

Passes

Passes

Passes

Passes

 
Packaging for Silica Gel - TLC Grade

 

FD – Fiber Drum - 25KG
 
C – Corrugated Box - 5 & 10 KG
 
 

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